产品货号：
GS2109
中文名称：
Tth111I内切酶
英文名称：
Tth111I
产品规格：
400U
发货周期：
1～3天
产品价格：
询价
储存条件：冷冻（-20℃）
使用说明
High enzyme concentration may result in star activity.
技术规格
相关搜索：Tth111I内切酶，Tth111I限制性内切酶
使用说明
High enzyme concentration may result in star activity.
技术规格
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1μgof Lambda DNA(HindIII-digest)in 1hourat 65℃ in a total reaction volume of 50μl. |
| Recognition sites | GACN↑NNGTC |
| 来源 | E.coli strain,that carries the cloned gene Tth111I from Thermus Thermophilus 111 |
| Assayed on | Lambda DNA(HindIII-digest) |
| Optimal buffer | Y?(33mMTris-acetate(pH 7.9 at 25℃);10 mM magnesium acetate;66 mM potassium acetate;1 mM DTT.) |
| Optimal temperature | 65℃ |
| Storage conditions | 10 mM Tris-HCl(pH 7.5);500 mM NaCl;0.1 mM EDTA;1 mM DTT;50%glycerol.Store at -20℃. |
| Ligations | After 2-fold overdigestion with enzyme 10%of the DNA fragments can be ligated. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μgof Lambda DNA with 5 u.a.of enzyme for 16hoursat 65℃. |
| Methlation effect | Methylation sensitivity not tested |
| Thermal inactivation | enzyme is inactivated by incubation at 80℃ for 20 mins. |
| Campatible Ends | AspI,PflFI,PsyI,TelI |
相关搜索：Tth111I内切酶，Tth111I限制性内切酶